The BS-MS Associate II, Biomarker Imaging will determine the cellular abundance and localization of biomarkers in situ using novel imaging modalities. The candidate will join a diverse team responsible for the delivery of high-quality biomarker data elucidating the mechanism of action and disease pathology in both genetic and pharmacologically induced pre-clinical models of human disease. This position will be instrumental in the group’s efforts to provide evidence of target engagement across the PTC Therapeutics pipeline with a particular interest in neurodegenerative programs. The incumbent will be responsible for communicating experimental results to his/her supervisor and working cross-functionally across multiple departments.

Primary responsibilities include:

• Independently carries out grossing, orienting, and cryo-embedding research tissues

• Maintain diagnostic viability of all specimens received and ensure correct labeling of all materials

• Responsible for cryo-sectioning and thaw-mounting fresh frozen tissues

• Perform rapid H&E or Cresyl Violet staining to quickly assess tissue coordinates and architecture

• Prepare and maintain tissue fixatives and reagents/solutions for staining of tissue sections

• Utilize an automated spray-coating device for high-resolution tissue derivatization and matrix applications compatible with imaging mass spectrometry

• Conduct IHC, ICC, and IF to determine the localization of biomarkers and/or pharmacological targets

• Scan and image stained slides for analysis (bright field, fluorescence and/or confocal)

• Perform digital image analysis using various computer programs such as FlexImaging or ImageJ

• Aid in non-histological experiments in support of in vitro and ex vivo biomarker quantitation for pre-clinical studies (e.g. tissue processing for quantitation of lipids, DNA oxidation, etc.)

• Keep records of laboratory protocols and maintenance of equipment in an ELN

• Demonstrates compliance with corporate, departmental, and job-specific requirements

• Bachelor’s degree in a scientific discipline with a minimum of 2 years of relevant experience

• Skilled at cryo-sectioning and thaw mounting fresh frozen tissues

• Experience with tissue and/or cell staining protocols

• Proficiency with Microsoft Office

• Excellent verbal and written communication and skills

• Analytical thinker with excellent problem-solving skills and the ability to adapt to changing priorities and deadlines 

• Excellent planning, organization and time management skills including the ability to support and prioritize multiple projects

• Previous experience with whole-body cryo-sectioning a plus

• Working knowledge of sample preparation for imaging mass spectrometry preferred